Bibtex @article{10.1093/plphys/kiab226, author = {Hu, Yang and Zhang, Zhiwu}, title = "{GridFree: A Python Package of Image Analysis for Interactive Grain Counting and Measuring}", journal = {Plant Physiology}, year = {2021}, month = {05}, issn = {0032-0889}, doi = {10.1093/plphys/kiab226}, url = {https://doi.org/10.1093/plphys/kiab226}, note = {kiab226}, eprint = {https://academic.oup.com/plphys/advance-article-pdf/doi/10.1093/plphys/kiab226/37929045/kiab226.pdf}, }

• install Python 3.6.5, download link: https://www.python.org/downloads/release/python-365/

• execute python3.6 -m pip install -r requirements.txt to install required packages

• execute ./run on a terminal to run the software

• execute python3.6 -m pip install -r requirements.txt to install required packages

• execute ./run on a terminal to run the software

• Download link: https://www.dropbox.com/s/nf6vsu1q5meb1ho/GridFree_winOS.zip?dl=0

• run cmd go to the path you downloaded GridFree, you can execute cd space your download path
• execute python -m pip install -r requirements.txt to install required packages
• execute python -m pip install opencv-python for opencv package
• execute python -m pip install scikit-image for package installation
• execute python -m pip install sklearn for package installation
• 
• YouTube: https://youtu.be/fSgH1-35Hyw

• execute python tkinterGUI_nw.py to run the software

Modify matplotlibrc file ADD: backend: TkAgg